Gaygamer.net has listed the 20 gayest video game characters ever!
My favorite on the list is #5: The King of the Cosmos! (Katamari Damacy)
Joachim (of Shadow Hearts fame) makes the list.
The list misses a few I would add, so here we go:
Isaac (Castlevania: Curse of Darkness)
When we first saw him, we all quoted Cartmen: "Gayer than gay come to gay town on the gay bus." He's sick and super cool, and very, very gay.
Keith (Shadow Hearts)
You see him surrounded by women and the conversations had with the girls classify him as heterosexual. But, man does he flame out when he gets the family sword. (Get your head out of the gutter!)
Walter (Castlevania: Lament of innocence)
This vamp mugged Tim Curry in a back alley for those fantastic boots. Everyone's first thought when they finally met Walter in the game was: Gaaaaaaaaaaaaaaaay!
And because I can't help myself:
Sephiroth (Final Fantasy 7)
He looks awesome in ways that can't be defined, but man, the gay jokes just roll of the tongue.